School of Medicine - Light Microscopy Facility
UNIVERSITY OF CALIFORNIA, SAN DIEGO

Hamamatsu NanoZoomer 2.0HT
Slide Scanning System



What it's good for

- Generating brightfield and multi-color fluorescent images of entire slides or
tissue sections automatically
- 3 fluorescent colors
- Fixed cells or sections on a standard 1 x 3 slide with a coverslip (#1.5)
- Can scan up to 210 slides automatically

What it's NOT good for

- Thick tissue slices
- Timelapse

Principles of operation

Fluorescent light from a mercury lamp is filtered for excitation and emission or brighfield light produce an image by scanning specified areas of a slide to generate montage reconstructions.

Technical Information

- 3 fluorescent color channels and brightfield:
       - DAPI
       - FITC
       - Texas Red
- Motorized X-Y capability for slide scanning
- Microscope objective:
      - 20x (.75 NA)